Genes And Their Effects On Gene Editing Essay - 1428 Words

Gene editing is one of the most researched fields of molecular biology, as manipulated genes deem possible studying the specific genes and their effects. In the past, there were several attempts to manipulate gene function, including homologous recombination, RNA interference (RNAi), zinc-finger nucleases (ZFNs) and transcription-activator like effector nucleases (TALENs). These past approaches are expensive and time-consuming to engineer as opposed to Crispr Cas9, limiting their widespread use. What the Crispr Cas9 technique offers, and biologists desire, is specificity: the ability to target and study particular DNA sequences in the expanse of a genome with ease of preparation as used in Fu et al. (2014) and Korkmaz et al. (2016). The Crispr Cas9 system requires only the redesign of the crRNA to change the target specificity. This aspect of the Crispr Cas9 differs from the other genome editing tools, including ZFNs and TALENs, in which engineering the protein-DNA interface is required. Furthermore, Crispr Cas9 enables rapid genome-wide search of gene function by generating large gRNA libraries. The rapid progress in developing Cas9 into a set of tools for molecular biology research has been remarkable most likely due to the simplicity, high efficiency and versatility of the technique. Of the designer nuclease systems currently available for precision genome engineering, the Crispr Cas9 system is by far the most user-friendly according to Korkmaz et al. (2016). In theirShow MoreRelatedDelivery Of The Cas Components1306 Words   |  6 Pagesmethods of transfection such as microinjection, gene gun, electroporation, sonoporation, and using viruses such as adenoviruses and lentiviruses. In cultured mammalian cells, researchers have used electroporation, nucleofection, and Lipofectamine mediated transfection methods to deliver vectors expressing the gene for gRNAs and Cas9 endonuclease. In cultured human and mouse cells, Lentiviral vectors have also been used as a mode of delivery of genes. In addition to animal models and cell lines, Cas9Read MoreGenetic Disorders ( Genetic Disease Foundation ) Essay1607 Words   |  7 Pagesrange from memory loss to blindness, physical abnormalities and more. A process known as gene editing was created in an attempt to do away with genetic disorders. Gene editing was named â€Å"Science Magazine s Breakthrough of the Year 2015† due to its ease and high accessibility (ScienceDirect). It works by using CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 to make changes in the genes of cells. Francisco Mojica discovered CRISPR in 1993 at the University of Alicante inRead MoreThe Consequences Of Gene Editing With CRISPR-Cas 91116 Words   |  5 Pages 4 Future Possibilities 4.1 Gene Editing with CRISPR-Cas 9 This technology was discovered in 2013 and very quickly applied to human cells in a laboratory setting. This technology has set the record in all the editing methods to control the genetic makeup of an offspring (Sas Lawrenz, 2017). This record is helped by the ability of CRISPR-Cas9 to replicate any gene in the human genome by using 20 bases. This method uses a RNA strand (CRISPR), the bases, and an endonuclease (Cas 9). The Cas 9 intentionallyRead MoreSingle Nucleotide Polymorphisms Essay1514 Words   |  7 Pagesmay be associated with genetic disease, most of which lie in non-coding regulatory elements. As only ~1% of human genes are protein coding, it may hardly be surprising that so many of these implicated features are found within non-coding regions. The task we must face now is to assess the impact and prevalence of each of these variants which may lead to breakthroughs in how disease genes are localised and mapped in different individuals and populations. GWAS is a powerful tool for identifying trendsRead MoreGreat Power, Great Responsibility1390 Words   |  6 PagesGreat Power, Great Responsibility Chances are you have or know someone that has a family member suffering from Alzheimer s disease. It’s effects are heartbreaking and awful for all involved, but what if Alzheimer s disappeared from the world? Due to recent technological innovations, gene editing could soon be used to cure genetic diseases like Alzheimer s. In the film Jurassic Park the character Dr. Ian Malcolm said â€Å"... scientists were so preoccupied with whether or not they could that theyRead MoreThe Use Of Crispr / Cas9 Gene Editing1507 Words   |  7 Pages The practical uses for CRISPR/Cas9 gene editing and other nuclease gene editing methods extend to animals as well. There are many proposed uses of the technology that need to be considered according to their associated risks and benefits. The first of which is the use of CRISPR to knock out genes associated with horn development in dairy cattle (Cima, 2016). Animals with horns present a hazard to animals kept in the same enclosure as them an d to the workers that handle them. Only a small percentageRead MoreA New Science Of Genetic Engineering Essay1130 Words   |  5 Pagesimpending future. He worried as to how the public would react to his life work. The growing potential of gene editing is sweeping the US, filled with both overwhelming hope as well as fear. This is a bridge which must be crossed to achieve trust within the biomedical community. To ensure the people this can bring great change without the unethical byproducts which could come with gene editing. Much of the American public is divided with the relatively new science of genetic engineering due to concerningRead MoreViable Capable To Be Used In An Experiment Essay1297 Words   |  6 Pages Viable- Capable to be used in an experiment Vitro fertilization- Creating embryos from a petri dish Germline editing- The act of revising the cells of an embryo Bioethicist- An individual who studies aspects of biology, such as gene editing Cleave- To cut or remove in a swift motion Genome- As said by the author of the article, Tina Hesman Saey, â€Å"...that make up the human genetic instruction book, or genome,† to paraphrase, a genome entails the genetic circumstances of an embryo or otherRead MoreI Am Kaylyn Stewart From The University Tech University1164 Words   |  5 PagesKaylyn Stewart One Gene at a Time Scene: Ted Talk I am Kaylyn Stewart from the KAS research center. I have a bachelors in biological science from Louisiana Tech University and I’m in the process of getting my masters in biomedical engineering. At KAS, we are currently gathering research on the world’s top new promising technology known as genome editing. Our goal at the KAS research center is to shine the light on the advantages and disadvantages of genome editing around the world and provideRead MoreGenome Editing Essay1614 Words   |  7 PagesGenome editing of germ line cells using CRISPR/Cas9 system Recent studies suggest that genome editing of germ line cells is an effective strategy for mutated gene correction in sperms and oocytes for the inhibition of onset of inherited disorder. The first time, germ line genome editing in human by CRISPR/Cas9 system , was carried out to correct mutation of HBB (Beta globin) gene in zygote of ÃŽ ²-thalassemia patients by homologous DNA recombination (HDR) [80, 81]. Embryonic genome editing utilized